Figure 4. Image of the CcoI antibody (green) used to stain MELAS (myoclonus, epilepsy, lactic acidosis and stroke like episodes) where a mutation in a mitochondrially-encoded tRNA gene alters the biogenesis of Cco. A noted difference in staining can be seen between cells which have the CcoI subunit and those which either have little to none of the subunit present. The antibody was used at 7.5 ug/mL of anti-Cco subunit 1 mAb (MS404) in 10% goat serum, overnight at 4°C, when testing fibroblasts or other adherent cells.

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