Figure 2. An example using MS133 to quantify Complex I levels using various concentration of mouse brain tissue.

Based on the above results, 13 µg of tissue extract was loaded to determine intra-assay reproducibility. (Note: for a statistical analysis it is preferred to use two dipsticks for each sample; intra-assay CV's are typically <=10%). The average of the signal intensities was determined, and then the amount (µg) of Complex I was interpolated off of the standard curve.

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