Figure 2. An example using MS131 to quantify Complex I levels using various concentration of human fibroblast extract.

Based on the above standard curve values, 13 µg of protein extract were loaded to each dipstick for the control sample (1) and the four unknown samples (2-5). Using GraphPad software, the signal intensity from the standard curve was interpolated and the quantity of Complex I in samples 1-5 was determined. Based on the above analysis, the unknown samples had 16% and 85% of Complex I levels compared to the control.

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