Figure 1. An example using MS131 to quantify Complex I levels using various concentration of human fibroblast extract.

Shown is a 1:2 dilution series using a positive control sample. Approximately 7 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example, the dilution series starts with 20 µg of fibroblast extract. A one-site hyperbola line was generated for best-fit analysis using GraphPad.

(click to close window)