Figure 1. Quantitative ELISA analysis of cytochrome c release from the mitochondria into the cytosol in HeLa cells induced to undergo apoptosis by Staurosporine treatment.

Cytosolic (C), mitochondrial (M) and nuclear (N) fractions of HeLa cells treated for 4 hrs with 0.00, 0.02, 0.06, 0.18, 0.54, 1.62, 4.86 and 14.58 µM Staurosporine (A and B) or with 0.0 and 1.0 µM Staurosporine (C, D, E) were prepared using Cell Fractionation Kit HT. Fractions, each derived from one well of a 96-well plate, were analyzed by Cytochrome c Protein Quantity Microplate Assay Kit (MSA41) (A, C and D). Parallel analyses of fractions prepared independently and thus representing inter-assay variation of the Cell Fractionation Kit HT are shown in C and D. Western blot analysis of cytochrome c using ApoTrack™™ Cytochrome c WB Antibody Cocktail (MSA12) is shown for comparison (B and E). Data represent mean +/- standard error of the mean, n=4 (A and C), n=3 (D), n=2 (E), n=1 (B).

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