Dicarbonyl/L-xylulose reductase (DCXR) monoclonal antibody
|
Catalog No. MS704
|
|
|
$325.00 - 100 µg
|
|
This antibody can also be purchased as part of a Sample Pack.
|
|
|
Product Specifications
|
|
|
Applications:
|
Western blotting, Immunocytochemistry, Immunoprecipitation, and In-Cell ELISA
|
|
Species Reactivity:
|
human
|
|
Host Species:
|
mouse
|
|
Isotype:
|
IgG1, k
|
|
Clone ID:
|
4G4AF5
|
|
Concentration:
|
1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
|
|
Suggested Working Concentration:
|
1 µg/mL for Western blotting
4 µg/mL for Immunocytochemistry
|
|
Storage Conditions:
|
4°C
|
|
Country of Origin:
|
USA
|
Top
|
|
|
|
|
WB Images
(click to enlarge)
Figure 1. Western blot analysis of isolated human liver mitochondria using MS704 anti-DCXR monoclonal antibody at the suggested working concentration of 1 ug/mL using a goat anti-mouse secondary conjugated to AP.
|
ICC Images
(click to enlarge)
Figure 2. Immunocytochemistry image of MS704 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (4G4AF5, 4 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane.
|
Top
|
|
|
|
|
Have you published studies using this product?
Let us know by filling out this form and recieve a $100 discount off your next order.
Top
|
|
|
|
CART
