Complex IV subunit IV monoclonal antibody for yeast

Catalog No. MS420

$325.00 - 100 µg




UniProt Number: P04037
Alternate Names: Cytochrome c oxidase subunit 4, Cytochrome c oxidase polypeptide IV, COX4, YGL187C, G1362
Structure and Function: In yeast Saccharomyces cerevisiae, cytochrome c oxidase (COX) is composed of 11 subunits, three of which are encoded in the mitochondrial genome (Cox1p, Cox2p and Cox3p). These three large subunits are transmembrane proteins that form the catalytic core of the enzyme.

The remaining subunits (Cox4p-Cox9p) are nuclear encoded and transported into the mitochondria after translation. Although the specific functions of the subunits are not completely understood, they are known to be involved in the assembly of the enzyme. Studies with yeast strains carrying null mutations in these subunits have shown complete loss of COX activity, suggesting that the subunits play a crucial role in assembly and stability of the enzyme. The COX assembly process is conserved between human and yeast.



 
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Product Specifications
 
Applications: Western blotting
Species Reactivity: S. cerevisiae
Host Species: mouse
Isotype: IgG1, κ
Clone ID: 1A12A12
Concentration: 1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 1 µg/mL for Western blotting
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA


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Figure 1. Mitochondria from yeast membrane extract (lane 1) detected with (MS420) anti-subunit IV (Cox4p) antibody.


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Downloadable Documents


   Technical Data Sheet

   Western blotting Protocol

   Complex IV Playbook

   MSDS Sodium Azide


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Published Studies Using This Product: Bella et al., 2010. Mutations in the mitochondrial protease gene AFG3L2 cause dominant hereditary ataxia SCA28.

Pan et al., 2009. Extension of chronological life span by reduced TOR signaling requires down-regulation of Sch9p and involves increased mitochondrial OXPHOS complex density.

Horan et al., 2005. Analysis of COX2 mutants reveals cytochrome oxidase subassemblies in yeast.

Vaena de Avalos et al., 2005. The phosphatidylglycerol/cardiolipin biosynthetic pathway is required for the activation of inositol phosphosphingolipid phospholipase C, Isc1p, during growth of Saccharomyces cerevisiae.


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