Complex IV subunit IV monoclonal antibody

Catalog No. MS408

$325.00 - 100 µg

UniProt Number: Isoform 1: P13073
Isoform 2: Q96KJ9
Alternate Names: Cytochrome c oxidase subunit 4 isoform 1, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cytochrome c oxidase polypeptide IV, COX4I1, Cytochrome c oxidase subunit 4 isoform 2, Cytochrome c oxidase subunit IV isoform 2, COX IV-2, COX4I2, COX4L2
Structure and Function: This protein is subunit 4 of the cytochrome c oxidase complex, which catalyzes the reduction of O2 to H2O as the final oxidase complex of the respiratory chain.
Disease Associations: Genetic alterations of this enzyme complex are a common cause of OXPHOS diseases and the enzyme is altered in patients with Alzheimer’s disease.


Product Specifications
Applications: Western blotting, Immunocytochemistry (heat-induced antigen-retrieval improves signal), Immunohistochemistry, Flow Cytometry
Species Reactivity: human, bovine
Host Species: mouse
Isotype: IgG2a, κ
Clone ID: 10G8D12C12
Concentration: 1 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 1 µg/mL for Western blotting
5 µg/mL for Immunocytochemistry
1 µg/mL for Flow Cytometry
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA



WB Images

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Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), and HepG2 (lane 3) detected with (MS408) anti-subunit IV antibody.

IHC Images

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Figure 2. Skeletal muscle immunohistochemistry using MS408- fixed frozen tissue sections from a patient with a single large deletion of the mtDNA. All muscle fibers express subunit IV of complex IV because this subunit is encoded by nuclear DNA and persists in a partial complex in the absence of mtDNA-encoded subunits. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.

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Figure 3. Colon immunohistochemistry tissue sections from a normal ageing patient show complex IV negative crypts due to clonal expansion of colonic stem cells bearing mutations in mtDNA that affect expression of complex IV. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).

ICC Images

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Figure 4. Mitochondrial localization of complex IV in normal human fibroblasts visualized by immunocytochemistry using anti-complex IV subunit IV mAb 10G8D12C12 (MS408). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with MS408 (10 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (1 µg/ml)

Flow Images

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Figure 5. HeLa cells were stained with 1 µg/mL Complex IV subunit IV antibody (MS408) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Immunocytochemistry Protocol

   Immunohistochemistry Protocol

   Flow Cytometry Protocol

   Complex IV Playbook

   MSDS Sodium Azide



Published Studies Using This Product: Schmidt et al., 2011. Effector granules in human T lymphocytes: proteomic evidence for two distinct species of cytotoxic effector vesicles.

Safdar et al., 2010. Aberrant mitochondrial homeostasis in the skeletal muscle of sedentary older adults.

McConell et al., 2010. Central role of nitric oxide synthase in AICAR and caffeine induced mitochondrial biogenesis in L6 myocytes.

Garibal et al., 2010. Caspase-8-mediated cleavage of Bid and protein phosphatase 2A-mediated activation of Bax are necessary for Verotoxin-1-induced apoptosis in Burkitt's lymphoma cells.

Kutuk et al., 2009. Cisplatin overcomes Bcl-2-mediated resistance to apoptosis via preferential engagement of Bak: critical role of Noxa-mediated lipid peroxidation.

Schmidt et al., 2009. Enrichment and analysis of secretory lysosomes from lymphocyte populations.

Hippe et al., 2008. Fas/CD95-mediated apoptosis of type II cells is blocked by Toxoplasma gondii primarily via interference with the mitochondrial amplification loop.

Arnoult, 2008. Apoptosis-associated mitochondrial outer membrane permeabilization assays.

Mazzucco et al., 2008. Entecavir for treatment of hepatitis B virus displays no in vitro mitochondrial toxicity or DNA polymerase gamma inhibition.

Trougakos et al., 2009. Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex.

Putignani et al., 2008. Alteration of expression levels of the oxidative phosphorylation system (OXPHOS) in breast cancer cell mitochondria.

Samavati et al., 2008. Tumor necrosis factor alpha inhibits oxidative phosphorylation through tyrosine phosphorylation at subunit I of cytochrome c oxidase.

Sibayama-Imazu et al., 2008. Induction of apoptosis in PA-1 ovarian cancer cells by vitamin K2 is associated with an increase in the level of TR3/Nur77 and its accumulation in mitochondria and nuclei.

Heyne et al., 2008. Resistance of mitochondrial p53 to dominant inhibition.

Lo et al., 2008. PGAM5 tethers a ternary complex containing Keap1 and Nrf2 to mitochondria.

Yee et al., 2008. Contribution of membrane localization to the apoptotic activity of PUMA.

Janssen et al., 2007. The A3243G tRNALeu(UUR) mutation induces mitochondrial dysfunction and variable disease expression without dominant negative acting translational defects in complex IV subunits at UUR codons.

Pravenec et al., 2007. Direct linkage of mitochondrial genome variation to risk factors for type 2 diabetes in conplastic strains.

Autret et al., 2007. Poliovirus induces Bax-dependent cell death mediated by c-Jun NH2-terminal kinase.

Kojima et al., 2007. Mitogen-activated protein kinase kinase inhibition enhances nuclear proapoptotic function of p53 in acute myelogenous leukemia cells.

Nakano et al., 2007. Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3.

Martin-Latil et al., 2007. Bax is activated during rotavirus-induced apoptosis through the mitochondrial pathway.

Kojima et al., 2006. Mdm2 inhibitor Nutlin-3a induces p53-mediated apoptosis by transcription-dependent and transcription-independent mechanisms and may overcome Atm-mediated resistance to fludarabine in chronic lymphocytic leukemia.

Kojima et al., 2005. MDM2 antagonists induce p53-dependent apoptosis in AML: implications for leukemia therapy.

Arnoult et al., 2005. Release of OPA1 during apoptosis participates in the rapid and complete release of cytochrome c and subsequent mitochondrial fragmentation.

Masuda et al., 2004. Involvement of tumor necrosis factor receptor-associated protein 1 (TRAP1) in apoptosis induced by beta-hydroxyisovalerylshikonin.


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