Complex IV subunit II (Cox2p) monoclonal antibody for yeast
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Catalog No. MS419
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$325.00 - 100 µg
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This antibody can also be purchased as part of a Sample Pack.
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UniProt Number:
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P00410
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Alternate Names:
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Cytochrome c oxidase subunit 2,
Cytochrome c oxidase polypeptide II
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Structure and Function:
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In yeast Saccharomyces cerevisiae, cytochrome c oxidase (COX) is composed of 11 subunits, three of which are encoded in the mitochondrial genome (Cox1p, Cox2p and Cox3p). These three large subunits are transmembrane proteins that form the catalytic core of the enzyme. Subunit 2 (Cox2p) of this core protein contains a copper A center that is involved in the transfer of electrons from cytochrome c oxidase to the bimetallic center of complex IV.
The remaining subunits (Cox4p-Cox9p) are nuclear encoded and transported into the mitochondria after translation. Although the specific functions of the subunits are not completely understood, they are known to be involved in the assembly of the enzyme. Studies with yeast strains carrying null mutations in these subunits have shown complete loss of COX activity, suggesting that the subunits play a crucial role in assembly and stability of the enzyme. The COX assembly process is conserved between human and yeast.
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Product Specifications
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Applications:
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Western blotting
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Species Reactivity:
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S. cerevisiae
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Host Species:
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mouse
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Isotype:
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IgG2a, κ
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Clone ID:
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4B12A5
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Concentration:
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1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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2 µg/mL for Western blotting
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Mitochondria from yeast membrane extract (lane 1) detected with (MS419) anti-subunit II (Cox2p) antibody.
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Published Studies Using This Product:
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Atkinson et al., 2010. Mzm1 influences a labile pool of mitochondrial zinc important for respiratory function.
Bella et al., 2010. Mutations in the mitochondrial protease gene AFG3L2 cause dominant hereditary ataxia SCA28.
Puchta et al., 2010. DMR1 (CCM1/YGR150C) of Saccharomyces cerevisiae encodes an RNA-binding protein from the pentatricopeptide repeat family required for the maintenance of the mitochondrial 15S ribosomal RNA.
Ilkow et al., 2010. The rubella virus capsid protein inhibits mitochondrial import.
Bestwick et al., 2010. The role of Coa2 in hemylation of yeast Cox1 revealed by its genetic interaction with Cox10.
Kucharczyk et al., 2009. Biochemical consequences in yeast of the human mitochondrial DNA 8993T>C mutation in the ATPase6 gene found in NARP/MILS patients.
Wang et al., 2008. Dominant membrane uncoupling by mutant adenine nucleotide translocase in mitochondrial diseases.
Bonawitz et al., 2007. Reduced TOR signaling extends chronological life span via increased respiration and upregulation of mitochondrial gene expression.
Rak et al., 2007. Yeast cells lacking the mitochondrial gene encoding the ATP synthase subunit 6 exhibit a selective loss of complex IV and unusual mitochondrial morphology.
Nouet et al., 2007. Rmd9p controls the processing/stability of mitochondrial mRNAs and its overexpression compensates for a partial deficiency of oxa1p in Saccharomyces cerevisiae.
Kirchman et al., 2007. Copper supplementation increases yeast life span under conditions requiring respiratory metabolism.
Krause-Buchholz et al., 2006. YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex.
Brandner et al., 2005. Taz1, an outer mitochondrial membrane protein, affects stability and assembly of inner membrane protein complexes: implications for Barth Syndrome.
Horan et al., 2005. Analysis of COX2 mutants reveals cytochrome oxidase subassemblies in yeast.
Hlavacek et al., 2005. The transcriptional activator HAP4 is a high copy suppressor of an oxa1 yeast mutation.
Rinehart et al., 2005. Saccharomyces cerevisiae imports the cytosolic pathway for Gln-tRNA synthesis into the mitochondrion.
Chevtzoff et al., 2005. The yeast cAMP protein kinase Tpk3p is involved in the regulation of mitochondrial enzymatic content during growth.
Lemaire et al., 2004. A yeast mitochondrial membrane methyltransferase-like protein can compensate for oxa1 mutations.
Kissová et al., 2004. Uth1p is involved in the autophagic degradation of mitochondria.
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