Complex IV subunit II monoclonal antibody

Catalog No. MS405

$325.00 - 100 µg

UniProt Number: P00403
Alternate Names: Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II, MT-CO2, COII, COXII, MTCO2
Structure and Function: Cytochrome c oxidase is the fourth complex in the respiratory chain and is responsible for catalyzing the conversion of O2 to H2O. Subunit 2 of the cytochrome c oxidase complex combines with two other subunits (1 and 3) to form a core protein structure that performs many functions of the enzyme. The metallic copper center of this subunit transfers electrons to the heme center of subunit 1, which results in the movement of electrons from cytochrome c to the heme A3 and copper B metallic center of complex IV.
Disease Associations: Defects in subunit 2 of cytochrome c oxidase can result in COX deficiency, which causes a wide range of symptoms from local myopathy to multiple system pathologies that begin between infancy and adulthood. Abnormalities in this subunit are also associated with tumor development.


Product Specifications
Applications: Western blotting, Immunocytochemistry (heat-induced antigen-retrieval improves signal), Flow Cytometry
Species Reactivity: human
Host Species: mouse
Isotype: IgG2a, κ
Clone ID: 12C4F12
Concentration: 1.0 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 1 µg/mL for Western blotting
5-10 µg/mL for Immunocytochemistry
1 µg/mL for Flow Cytometry
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA



WB Images

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Figure 1. Isolated mitochondria from human heart (lane 1) detected with (MS405) anti-subunit ii antibody.

ICC Images

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Figure 2. Mitochondrial localization of complex IV visualized by immunocytochemistry using anti-complex IV subunit II mAb 12C4F12 (MS405). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with MS405 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (2 µg/ml).

Flow Images

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Figure 3. HeLa cells were stained with 1 µg/mL Complex IV subunit II antibody (MS405) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Immunocytochemistry Protocol

   Flow Cytometry Protocol

   Complex IV Playbook

   MSDS Sodium Azide



Published Studies Using This Product: Ferreira et al., 2011. Progressive cavitating leukoencephalopathy associated with respiratory chain complex I deficiency and a novel mutation in NDUFS1.

Kemp et al., 2010. Nuclear factors involved in mitochondrial translation cause a subgroup of combined respiratory chain deficiency.

Leadsham et al., 2010. cAMP/PKA signaling balances respiratory activity with mitochondria dependent apoptosis via transcriptional regulation.

Gegg et al., 2010. Mitofusin-1 and Mitofusin-2 are ubiquitinated in a PINK1/parkin dependent manner upon induction of mitophagy.

Fassone et al., 2010. FOXRED1, encoding a FAD-dependent oxidoreductase complex-I specific molecular chaperone, is mutated in infantile-onset mitochondrial encephalopathy.

Escobar-Alvarez et al., 2010. Inhibition of Human Peptide Deformylase Disrupts Mitochondrial Function.

Nadanaciva et al., 2010. High-Content Screening for Compounds That Affect mtDNA-Encoded Protein Levels in Eukaryotic Cells.

Safdar et al., 2010. Aberrant mitochondrial homeostasis in the skeletal muscle of sedentary older adults.

Wydro et al., 2010. Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes mitochondrial translation inhibition.

Fogal et al., 2010. Mitochondrial p32 Protein is a Critical Regulator of Tumor Metabolism via Maintenance of Oxidative Phosphorylation.

Khalimonchuk et al., 2010. Formation of the redox cofactor centers during Cox1 maturation in yeast cytochrome oxidase.

Fogal et al., 2010. Mitochondrial p32 Protein is a Critical Regulator of Tumor Metabolism via Maintenance of Oxidative Phosphorylation.

Bestwick et al., 2010. The role of Coa2 in hemylation of yeast Cox1 revealed by its genetic interaction with Cox10.

Kozie? et al., 2009. Plasma membrane associated membranes (PAM) from Jurkat cells contain STIM1 protein is PAM involved in the capacitative calcium entry?

Bellance et al., 2009. Bioenergetics of lung tumors: alteration of mitochondrial biogenesis and respiratory capacity.

Horvath et al., 2009. Molecular basis of infantile reversible cytochrome c oxidase deficiency myopathy.

Rackham et al., 2009. Pentatricopeptide repeat domain protein 1 lowers the levels of mitochondrial leucine tRNAs in cells.

Leshinsky-Silver et al., 2009. NDUFS4 mutations cause Leigh syndrome with predominant brainstem involvement.

Crouser et al., 2009. Monocyte activation by necrotic cells is promoted by mitochondrial proteins and formyl peptide receptors.

Villarroya et al., 2009. Altered gene transcription profiles in fibroblasts harboring either TK2 or DGUOK mutations indicate compensatory mechanisms.

Gegg et al., 2009. Silencing of PINK1 expression affects mitochondrial DNA and oxidative phosphorylation in dopaminergic cells.

Calvaruso et al., 2008. Electrophoresis techniques to investigate defects in oxidative phosphorylation.

Hakonen et al., 2008. Infantile-onset spinocerebellar ataxia and mitochondrial recessive ataxia syndrome are associated with neuronal complex I defect and mtDNA depletion.

Leroy et al., 2008. Protective effect of eicosapentaenoic acid on palmitate-induced apoptosis in neonatal cardiomyocytes.

Yeo et al., 2008. Skeletal muscle adaptation and performance responses to once a day versus twice every second day endurance training regimens.

Khidr et al., 2008. Role of SUV3 helicase in maintaining mitochondrial homeostasis in human cells.

Rorbach et al., 2008. The human mitochondrial ribosome recycling factor is essential for cell viability.

Pierrel et al., 2008. Coa2 is an assembly factor for yeast cytochrome c oxidase biogenesis that facilitates the maturation of Cox1.

Fornuskova et al., 2008. The impact of mitochondrial tRNA mutations on the amount of ATP synthase differs in the brain compared to other tissues.

Balasubramanian et al., 2008. A novel histone deacetylase 8 (HDAC8)-specific inhibitor PCI-34051 induces apoptosis in T-cell lymphomas.

Caron et al., 2008. Contribution of mitochondrial dysfunction and oxidative stress to cellular premature senescence induced by antiretroviral thymidine analogues.

Pagel-Langenickel et al., 2007. A discordance in rosiglitazone mediated insulin sensitization and skeletal muscle mitochondrial content/activity in Type 2 diabetes mellitus.

Margineantu et al., 2007. Hsp90 inhibition decreases mitochondrial protein turnover.

Pierrel et al., 2007. Coa1 links the Mss51 post-translational function to Cox1 cofactor insertion in cytochrome c oxidase assembly.

Janssen et al., 2007. The A3243G tRNALeu(UUR) mutation induces mitochondrial dysfunction and variable disease expression without dominant negative acting translational defects in complex IV subunits at UUR codons.

Ranney et al., 2007. Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer.

Pravenec et al., 2007. Direct linkage of mitochondrial genome variation to risk factors for type 2 diabetes in conplastic strains.

Lebon et al., 2007. A novel mutation of the NDUFS7 gene leads to activation of a cryptic exon and impaired assembly of mitochondrial complex I in a patient with Leigh syndrome.

Fujita et al., 2007. CHOP (C/EBP homologous protein) and ASNS (asparagine synthetase) induction in cybrid cells harboring MELAS and NARP mitochondrial DNA mutations.

Gibala et al., 2006. Short-term sprint interval versus traditional endurance training: similar initial adaptations in human skeletal muscle and exercise performance.

Pye et al., 2006. Production of transmitochondrial cybrids containing naturally occurring pathogenic mtDNA variants.

Mazzanti et al., 2006. Differential expression proteomics of human colon cancer.

Soucek et al., 2006. Transforming growth factor-beta1 inhibits all-trans retinoic acid-induced apoptosis.

Mazzanti et al., 2006. Coordination of nuclear- and mitochondrial-DNA encoded proteins in cancer and normal colon tissues.

Clohessy et al., 2006. Mcl-1 interacts with truncated Bid and inhibits its induction of cytochrome c release and its role in receptor-mediated apoptosis.

Schrauwen et al., 2006. Reduced skeletal muscle uncoupling protein-3 content in prediabetic subjects and type 2 diabetic patients: restoration by rosiglitazone treatment.

Stiburek et al., 2005. Tissue-specific cytochrome c oxidase assembly defects due to mutations in SCO2 and SURF1.

Stankiewicz et al., 2005. Hsp70 inhibits heat-induced apoptosis upstream of mitochondria by preventing Bax translocation.

Sadacharan et al., 2005. Localization of mitochondrial DNA encoded cytochrome c oxidase subunits I and II in rat pancreatic zymogen granules and pituitary growth hormone granules.

Jiang et al., 2005. Role of Smac/DIABLO in hydrogen peroxide-induced apoptosis in C2C12 myogenic cells.

Jiang et al., 2005. Heat shock pretreatment inhibited the release of Smac/DIABLO from mitochondria and apoptosis induced by hydrogen peroxide in cardiomyocytes and C2C12 myogenic cells.

Rose et al., 2005. Beta-phenylethyl isothiocyanate mediated apoptosis; contribution of Bax and the mitochondrial death pathway.

Miró et al., 2004. Upregulatory mechanisms compensate for mitochondrial DNA depletion in asymptomatic individuals receiving stavudine plus didanosine.

Gianni et al., 2004. Oxidative stress and the mitochondrial theory of aging in human skeletal muscle.

Cisar et al., 2004. Differential expression of mitochondrial electron transport chain proteins in cardiac tissues of broilers from pulmonary hypertension syndrome-resistant and -susceptible lines.

Cheung et al., 2004. Lactacystin-induced apoptosis of cultured mouse cortical neurons is associated with accumulation of PTEN in the detergent-resistant membrane fraction.

Guo et al., 2004. Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death.

Chen et al., 2004. Mitochondrial localization of ERalpha and ERbeta in human MCF7 cells.


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