Complex IV subunit I (Cox1p) monoclonal antibody for yeast

Catalog No. MS418

$325.00 - 100 µg

UniProt Number: P00401
Alternate Names: Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I, COX1, OXI3, Q0045
Structure and Function: In yeast Saccharomyces cerevisiae, cytochrome c oxidase (COX) is composed of 11 subunits, three of which are encoded in the mitochondrial genome (Cox1p, Cox2p and Cox3p). These three large subunits are transmembrane proteins that form the catalytic core of the enzyme. Subunit 1 (Cox1p) of this core protein contains a heme A group that is involved in the transfer of electrons from cytochrome c oxidase to the bimetallic center of complex IV.

The remaining subunits (Cox4p-Cox9p) are nuclear encoded and transported into the mitochondria after translation. Although the specific functions of the subunits are not completely understood, they are known to be involved in the assembly of the enzyme. Studies with yeast strains carrying null mutations in these subunits have shown complete loss of COX activity, suggesting that the subunits play a crucial role in assembly and stability of the enzyme. The COX assembly process is conserved between human and yeast.


Product Specifications
Applications: Western blotting
Species Reactivity: S. cerevisiae
Host Species: mouse
Isotype: IgG2b, κ
Clone ID: 11D8B7
Concentration: 1 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 3 µg/mL for Western blotting
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA


WB Images

(click to enlarge)

Figure 1. Mitochondria from yeast membrane extract (lane 1) detected with (MS418) anti-subunit I (Cox1p) antibody.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Complex IV Playbook

   MSDS Sodium Azide



Published Studies Using This Product: Atkinson et al., 2010. Mzm1 influences a labile pool of mitochondrial zinc important for respiratory function.

Bella et al., 2010. Mutations in the mitochondrial protease gene AFG3L2 cause dominant hereditary ataxia SCA28.

Perez-Martinez et al., 2009. Dual functions of Mss51 couple synthesis of Cox1 to assembly of cytochrome c oxidase in Saccharomyces cerevisiae mitochondria.

Rak et al., 2007. Yeast cells lacking the mitochondrial gene encoding the ATP synthase subunit 6 exhibit a selective loss of complex IV and unusual mitochondrial morphology.

Horan et al., 2005. Analysis of COX2 mutants reveals cytochrome oxidase subassemblies in yeast.


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