MitoSciences: MS431 Complex IV Human Protein Quantity Dipstick Assay Kit

Enzymes > Complex IV > Complex IV Human Protein Quantity Dipstick Assay Kit

Complex IV Human Protein Quantity Dipstick Assay Kit

Catalog No. MS431

$325.00 - 30 tests
$595.00 - 90 tests

Product Description

Contains 30 or 90 dipsticks and necessary components to quantify the levels of the fully assembled cytochrome c oxidase enzyme complex from human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

Learn more about Dipstick Protein Quantity Assays >

Specifications

Data

Documentation

Citations

Product Specifications
Species Reactivity:	human, bovine
Storage Conditions:	Store dipsticks and gold-conjugated secondary antibody at room temperature out of direct sunlight in their provided containers. Store buffers at 4° C.
Country of Origin:	USA

Top

Specifications

Data

Documentation

Citations

Figure 1

(click to enlarge)
Figure 1. An example using MS431 to quantify Complex IV levels using various concentration of human fibroblast extract.

Shown is a 1:2 dilution series using a positive control sample. Approximately 7 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 10 µg of fibroblast extract. A one-site hyperbola line was generated for best-fit analysis using GraphPad.

Figure 2

(click to enlarge)
Figure 2. An example using MS431 to quantify Complex IV levels using various concentration of human fibroblast extract.

Based on the above standard curve values, 5 µg of protein extract were loaded to each dipstick for the four unknown samples (1-4). Using GraphPad software, the signal intensity from the standard curve was interpolated and the quantity of Complex I in samples 1-5 was determined. Based on the above analysis, the unknown samples had between 4% and 64% of Complex IV levels compared to the control.

Top