Products for Complex IV (cytochrome c oxidase)
- Microplate Assay Kits
- Dipsticks Assay Kits
- Research Kits
- Individual Antibodies
Overview
Complex IV, also called cytochrome c oxidoreductase or cytochrome c oxidase, is a complex of 13 different subunits, 3 of which (I, II and III) are encoded on mtDNA, with the remainder nuclear encoded. The complex contains 2 heme groups (a and a3) and 2 copper atoms as prosthetic groups. Genetic alterations of this enzyme complex are a common cause of OXPHOS diseases. The enzyme is altered in Alzheimers disease. Also there are reports of reduced amounts of this complex in cancer cells when these cells become hypoxic.
Rapid Microplate Assay Kits
MS441 MitoProfile® Rapid Microplate Assay Kit for Human Complex IV Activity
A rapid activity microplate kit that is used to determine the activity of cytochrome c oxidase in a human sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS442 MitoProfile® Rapid Microplate Assay Kit for Human Complex IV Quantity
A rapid activity microplate kit that is used to determine the amount of cytochrome c oxidase in a human sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS443 MitoProfile® Rapid Microplate Assay Kit for Human Complex IV Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both the activity and quantity of cytochrome c oxidase in a human sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS444 MitoProfile® Rapid Microplate Assay Kit for Mouse Complex IV Activity
A 96-well activity microplate kit that is used to determine the activity of cytochrome c oxidase in a mouse sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS445 MitoProfile® Rapid Microplate Assay Kit for Mouse Complex IV Quantity
A 96-well activity microplate kit that is used to determine the amount of cytochrome c oxidase in a mouse sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS446 MitoProfile® Rapid Microplate Assay Kit for Mouse Complex IV Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both the activity and quantity of cytochrome c oxidase in a mouse sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS447 MitoProfile® Rapid Microplate Assay Kit for Rat Complex IV Activity
A 96-well activity microplate kit that is used to determine the activity of cytochrome c oxidase in a rat sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Dipstick Assay Kits
MS430 MitoProfile® Dipstick Assay Kit for Human Complex IV Activity
Contains 30 or 60 dipsticks and necessary components for quantitative analysis of cytochrome c oxidase activity in human and bovine samples.
MS431 MitoProfile® Dipstick Assay Kit for Human Complex IV Quantity
Contains 30 or 60 dipsticks and necessary components for quantitative analysis of cytochrome c oxidase in human and bovine samples.
MS432 MitoProfile® Dipstick Assay Kit for Rodent Complex IV Activity
Contains 30 or 60 dipsticks and necessary components for quantitative analysis of cytochrome c oxidase activity in mouse and rat samples.
Research Kits
MS401 MitoProfile® Complex IV Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.
MS402 MitoProfile® Complex IV Immunocapture Kit for Rodent
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria. The Complex IV immunocapture kit allows isolation of the cytochrome c oxidase complex (E.C. 1.9.3.1) from small amounts of rat or mouse tissue.
MS601 MitoProfile® Human Total OXPHOS Complexes Detection Kit
Contains a total of 360 µg or 720 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western blotting control.
MS602 MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
A cocktail of 220 µg antibodies comprised of six mAbs packaged individually. Each antibody is against a labile subunit of each of the 5 OXPHOS complexes and PDH. Each mAb is provided in a 50 µl aliquot at a 200x concentration, to allow reconstitution of each mAb in 10 ml working volume.
MS602a MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
Includes the same components as MS602 (above), but additionally contains 60 µg mAb against porin, and 120 µg against Complex V α subunit, and the required secondary antibodies for simultaneous 2 dye staining of samples.
MS604 MitoProfile® Rodent Total OXPHOS Complexes Detection Kit
Contains a total of 300 µg or 600 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from rodent sources. Mouse brain mitochondria are also included as a Western blotting control.
Individual Antibodies
MS401c Complex IV Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture ~10 µg of Complex IV from heart mitochondria.
MS403 Complex IV subunit SURF1 monoclonal antibody
100 µg of monoclonal antibody reactive against the 33 kDa Complex IV assembly factor SURF1. Cellular levels of SURF1 are highest in growing cultured cells.
MS404 Complex IV subunit I monoclonal antibody
100 µg of monoclonal antibody reactive against the 57 kDa COX (Complex IV) subunit I. The apparent molecular weight is 35 kDa. MS404 also labels COX I in immunocytochemical applications.
MS405 Complex IV subunit II monoclonal antibody
100 µg of monoclonal antibody reactive against the 26 kDa COX (Complex IV) subunit II. MS405 also labels COX II in immunocytochemical applications.
MS406 Complex IV subunit III monoclonal antibody
100 µg of monoclonal antibody reactive against the 30 kDa COX (Complex IV) subunit III. The apparant molecular weight is 22 kDa.
MS407 Complex IV subunit IV monoclonal antibody
100 µg of monoclonal antibody reactive against the 18 kDa COX (Complex IV) subunit IV. Reacts with human, bovine, mouse and rat. MS408 is an alternative antibody against COX IV.
MS408 Complex IV subunit IV monoclonal antibody (alternate to MS407)
100 µg of monoclonal antibody reactive against the 18 kDa COX (Complex IV) subunit IV. MS408 also labels COX IV in immunocytochemical applications. Reacts only with human and bovine, but better than MS407 for immunocytochemistry in these species.
MS409 Complex IV subunit Va monoclonal antibody
100 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit Va. MS409 also labels COX Va in immunocytochemical applications.
MS410 Complex IV subunit Vb monoclonal antibody
100 µg of monoclonal antibody reactive against the 13 kDa COX (Complex IV) subunit Vb.
MS411 Complex IV subunit VIaH monoclonal antibody
250 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit VIa and specific to the 'heart' isoform.
MS412 Complex IV subunit VIaL monoclonal antibody
100 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit VIa and specific to the 'liver' isoform.
MS413 Complex IV subunit VIb monoclonal antibody
100 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit VIb.
MS414 Complex IV subunit VIc monoclonal antibody
100 µg of monoclonal antibody reactive against the 12 kDa COX (Complex IV) subunit VIc.
MS415 Complex IV subunit VIIaHL monoclonal antibody
250 µg of monoclonal antibody reactive against the 8 kDa COX (Complex IV) subunit VIIa and reacting against both 'liver' and 'heart' isoforms.
MS416 Complex IV subunit VIIb monoclonal antibody
250 µg of monoclonal antibody reactive against the 8 kDa COX (Complex IV) subunit VIIb.
MS418 Complex IV subunit I monoclonal antibody for yeast
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit I in Saccharomyces cerevisiae for use in Western blotting applications.
MS419 Complex IV subunit II monoclonal antibody for yeast
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit II in Saccharomyces cerevisiae for use in Western blotting applications.
MS420 Complex IV subunit IV monoclonal antibody for yeast
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit IV in Saccharomyces cerevisiae for use in Western blotting applications.
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Featured
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The Complex IV Playbook
Download our free guide to conducting research into cytochrome c oxidase. Original research data and figures from MitoSciences scientists.
Complex IV deficiency has been implicated in diseases such as Alzheimer's Disease and cancer.
Learn More
Summary of Recent Research
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CART
