Products for Complex III
 (ubiquinol cytochrome c oxidoreductase)


- Research Kits
- Individual Antibodies

Overview

Complex III, or ubiquinol cytochrome c oxidoreductase, is a complex of 11 subunits and catalyses electron transfer from reduced ubiquinone or coenzyme Q 10 to cytochrome c. The complex contains 11 different subunits, one of which, cytochrome b, is encoded on mtDNA, the remainder are nuclear encoded. Genetic complex III deficiencies occur but are rare

 

Research Kits


MS301 MitoProfile® Complex III Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.

MS601 MitoProfile® Human Total OXPHOS Complexes Detection Kit
Contains a total of 360 µg or 720 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western blotting control.

MS602 MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
A cocktail of 220 µg antibodies comprised of six mAbs packaged individually. Each antibody is against a labile subunit of each of the 5 OXPHOS complexes and PDH. Each mAb is provided in a 50 µl aliquot at a 200x concentration, to allow reconstitution of each mAb in 10 ml working volume.

MS602a MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
Includes the same components as MS602 (above), but additionally contains 60 µg mAb to porin, and 120 µg to Complex V α subunit, and the required secondary antibodies for simultaneous 2 dye staining of samples.

MS604 MitoProfile® Rodent Total OXPHOS Complexes Detection Kit
Contains a total of 300 µg or 600 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from mouse sources. Mouse brain mitochondria are also included as a Western blotting control.


 

Individual Antibodies


MS301c Complex III Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture ~10 g of Complex III from heart mitochondria.

MS303 Complex III subunit Core 1 monoclonal antibody
100 µg of monoclonal antibody reactive against the 49 kDa complex III Core protein 1.

MS304 Complex III subunit Core 2 monoclonal antibody
100 µg of monoclonal antibody reactive against the 47 kDa Complex III Core protein 2. MS304 also labels core 2 in immunocytochemical applications.

MS305 Complex III subunit Rieske monoclonal antibody
100 µg of monoclonal antibody reactive against the 22 kDa complex III Rieske subunit.

MS306 Complex III subunit 10 kDa monoclonal antibody
100 µg of monoclonal antibody reactive against the complex III 10 kDa subunit.


Featured            
Product
MS301 Complex III Immunocapture Kit

This kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex (E.C. 1.10.2.2) from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging.

Uses for the Complex III immunocapture kit include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.
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