MitoSciences: MS133 Complex I Rodent Protein Quantity Dipstick Assay Kit

Enzymes > Complex I > Complex I Rodent Protein Quantity Dipstick Assay Kit

Complex I Rodent Protein Quantity Dipstick Assay Kit

Catalog No. MS133

$325.00 - 30 tests
$595.00 - 90 tests

Product Description

Contains 30 or 90 dipsticks and necessary components to quantify the levels of the fully assembled Complex I enzyme complex from mouse and rat samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

Learn more about Dipstick Protein Quantity Assays >

Specifications

Data

Documentation

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Product Specifications
Species Reactivity:	mouse, rat
Storage Conditions:	Store dipsticks and gold-conjugated secondary antibody at room temperature out of direct sunlight in their provided containers. Store buffers at 4°C.
Country of Origin:	USA

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Specifications

Data

Documentation

Citations

Figure 1

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Figure 1. An example using MS133 to quantify Complex I levels using various concentration of mouse brain tissue.

Shown is a 1:2 dilution series using a positive control sample. Approximately 6 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 20 µg of mouse brain tissue extract. A one-site hyperbola line was generated for best-fit analysis using GraphPad.

Figure 2

(click to enlarge)
Figure 2. An example using MS133 to quantify Complex I levels using various concentration of mouse brain tissue.

Based on the above results, 13 µg of tissue extract was loaded to determine intra-assay reproducibility. (Note: for a statistical analysis it is preferred to use two dipsticks for each sample; intra-assay CV's are typically <=10%). The average of the signal intensities was determined, and then the amount (µg) of Complex I was interpolated off of the standard curve.

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