Complex I subunit NDUFA9 monoclonal antibody

Catalog No. MS111

$325.00 - 100 µg

UniProt Number: Q16795
Alternate Names: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, NADH-ubiquinone oxidoreductase 39 kDa subunit, Complex I-39kD, CI-39kD, NDUFA9, NDUFS2L
Structure and Function: There are 45 unique subunits of Complex I, and this subunit is 377 AA long. Each NDUFA9 subunit can bind one FAD molecule. It is thought that this subunit does not contribute to catalytic functions of Complex I.


Product Specifications
Applications: Western blotting, Immunohistochemistry, Blue Native Electrophoresis
Species Reactivity: human, mouse, rat, bovine
Host Species: mouse
Isotype: IgG1, κ
Clone ID: 20C11B11B11
Concentration: 1 mg/mL in Hepes-Buffered Saline (HBS)-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 1 µg/mL for Western blotting
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA


WB Images

(click to enlarge)

Figure 1. Isolated mitochondria from human heart (lane 1), bovine heart (lane 2), rat heart (lane 3), and mouse heart (lane 4) detected with (MS111) anti-NDUFA9 antibody. Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
IHC Images

(click to enlarge)

Figure 2. Human spinal column tissue, formalin fixed and paraffin embedded, and antibody detection with MS111 using the ABC system. MS111 diluted 1:100 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1:100 in 4% BSA in PBST) for 1 hour at room temp. Washed x3 in PBST and peroxidase activity was demonstrated using kit from Vector. Antigen retrieval was performed by 1 min pressure cooking 1mmol EDTA pH8.



Downloadable Documents

   Technical Data Sheet

   Western blotting Protocol

   Immunohistochemistry Protocol

   Blue Native Electrophoresis Protocol

   MSDS Sodium Azide



Published Studies Using This Product: Ferreira et al., 2011. Progressive cavitating leukoencephalopathy associated with respiratory chain complex I deficiency and a novel mutation in NDUFS1.

Strub et al., 2010. Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration.

Gegg et al., 2010. Mitofusin-1 and Mitofusin-2 are ubiquitinated in a PINK1/parkin dependent manner upon induction of mitophagy.

Fassone et al., 2010. FOXRED1, encoding a FAD-dependent oxidoreductase complex-I specific molecular chaperone, is mutated in infantile-onset mitochondrial encephalopathy.

Tuppen et al., 2010. The p.M292T NDUFS2 mutation causes complex I-deficient Leigh syndrome in multiple families.

Thomas et al., 2010. Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin.

Dennerlein et al., 2010. Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit.

Mayr et al., 2010. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 {varepsilon} subunit.

Berger et al., 2010. Membrane localization of LRRK2 is associated with increased formation of the highly active LRRK2 dimer and changes in its phosphorylation.

Ylikallio et al., 2010. High mitochondrial DNA copy number has detrimental effects in mice.

Ahola-Erkkila et al., 2010. Ketogenic diet slows down mitochondrial myopathy progression in mice.

Wydro et al., 2010. Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes mitochondrial translation inhibition.

Baracca et al., 2010. Mitochondrial Complex I decrease is responsible for bioenergetic dysfunction in K-ras transformed cells.

Kachadourian et al., 2009. Casiopeína IIgly-induced oxidative stress and mitochondrial dysfunction in human lung cancer A549 and H157 cells.

Sheftel et al., 2009. Human ind1, an iron-sulfur cluster assembly factor for respiratory complex I.

Area-Gomez et al., 2009. Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria.

Gómez et al., 2009. Supercomplexes of the mitochondrial electron transport chain decline in the aging rat heart.

Arthur et al., 2009. Parkinson's disease brain mitochondria have impaired respirasome assembly, age-related increases in distribution of oxidative damage to mtDNA and no differences in heteroplasmic mtDNA mutation abundance.

Ogawa et al., 2009. Heterogeneous levels of oxidative phosphorylation enzymes in rat adrenal glands.

Rackham et al., 2009.Pentatricopeptide repeat domain protein 1 lowers the levels of mitochondrial leucine tRNAs in cells.

Grünewald et al., 2009. Differential effects of PINK1 nonsense and missense mutations on mitochondrial function and morphology.

Keeney et al., 2009. Mitochondrial gene therapy augments mitochondrial physiology in a Parkinson's disease cell model.

Gerards et al., 2009. Defective complex I assembly due to C20orf7 mutations as a new cause of Leigh syndrome.

Iyer et al., 2009. Recombinant mitochondrial transcription factor A with N-terminal mitochondrial transduction domain increases respiration and mitochondrial gene expression.

Galati et al., 2009. Role of nuclear-encoded subunit Vb in the assembly and stability of cytochrome c oxidase complex: implications in mitochondrial dysfunction and ROS production.

Palmfeldt et al., 2009. Mitochondrial proteomics on human fibroblasts for identification of metabolic imbalance and cellular stress.

Vempati et al., 2009. Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV.

Hu et al., 2009. Increased enzymatic O-GlcNAcylation of mitochondrial proteins impairs mitochondrial function in cardiac myocytes exposed to high glucose.

Missihoun et al., 2009. Myocardial oxidative stress, osteogenic phenotype, and energy metabolism are differentially involved in the initiation and early progression of delta-sarcoglycan-null cardiomyopathy.

Leary et al., 2009. Oxidative phosphorylation: synthesis of mitochondrially encoded proteins and assembly of individual structural subunits into functional holoenzyme complexes.

Hakonen et al., 2008. Infantile-onset spinocerebellar ataxia and mitochondrial recessive ataxia syndrome are associated with neuronal complex I defect and mtDNA depletion.

Calvaruso et al., 2008. Electrophoresis techniques to investigate defects in oxidative phosphorylation.

Cooper et al., 2008. Modulation of PGC-1 coactivator pathways in brown fat differentiation through LRP130.

Minai et al., 2008. Mitochondrial respiratory chain complex assembly and function during human fetal development.

Son et al., 2008. Isolated cytochrome c oxidase deficiency in G93A SOD1 mice overexpressing CCS protein.

Fornuskova et al., 2008. The impact of mitochondrial tRNA mutations on the amount of ATP synthase differs in the brain compared to other tissues.

Mayr et al., 2008. Loss of complex I due to mitochondrial DNA mutations in renal oncocytoma.

Devi et al., 2008. Mitochondrial import and accumulation of alpha-synuclein impair complex I in human dopaminergic neuronal cultures and Parkinson disease brain.

Lebon et al., 2007. A novel mutation of the NDUFS7 gene leads to activation of a cryptic exon and impaired assembly of mitochondrial complex I in a patient with Leigh syndrome.

Pravenec et al., 2007. Direct linkage of mitochondrial genome variation to risk factors for type 2 diabetes in conplastic strains.

Li et al., 2007. An assembled complex IV maintains the stability and activity of complex I in mammalian mitochondria.

Park et al., 2007. Yeast NDI1 improves oxidative phosphorylation capacity and increases protection against oxidative stress and cell death in cells carrying a Leber's hereditary optic neuropathy mutation.

Cheung et al., 2006. Dissociating the dual roles of apoptosis-inducing factor in maintaining mitochondrial structure and apoptosis.

Pye et al., 2006. Production of transmitochondrial cybrids containing naturally occurring pathogenic mtDNA variants.

Wang et al., 2006. Human mitochondrial DNA nucleoids are linked to protein folding machinery and metabolic enzymes at the mitochondrial inner membrane.

Gybina et al., 2006. Variable response of selected cuproproteins in rat choroid plexus and cerebellum following perinatal copper deficiency.

Leshinsky-Silver et al., 2005. Fulminant neurological deterioration in a neonate with Leigh syndrome due to a maternally transmitted missense mutation in the mitochondrial ND3 gene.

Schilling et al., 2005. Rapid purification and mass spectrometric characterization of mitochondrial NADH dehydrogenase (Complex I) from rodent brain and a dopaminergic neuronal cell line.


Browse Products By:
Product Search:

or use our     
Product Finder Tool   

Sales & Customer Support:

[email protected]

© 2004-2011 MitoSciences Inc, an Abcam company. All rights reserved.