Enzymes > Complex I > Complex I Human Protein Quantity Dipstick Assay Kit

Complex I Human Protein Quantity Dipstick Assay Kit

Catalog No. MS131

$325.00 - 30 tests
$595.00 - 90 tests


Product Description

Contains 30 or 90 dipsticks and necessary components to quantify the levels of the fully assembled Complex I enzyme complex in human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

Learn more about Dipstick Protein Quantity Assays >



Specifications
 
Data
 
Documentation
 
Citations

Product Specifications
  
Species Reactivity: human, bovine
Storage Conditions: Store dipsticks and gold-conjugated secondary antibody at room temperature out of direct sunlight in their provided containers. Store buffers at 4°C.
Country of Origin: USA


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Specifications
 
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Documentation
 
Citations

Figure 1


(click to enlarge)

Figure 1. An example using MS131 to quantify Complex I levels using various concentration of human fibroblast extract.

Shown is a 1:2 dilution series using a positive control sample. Approximately 7 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example, the dilution series starts with 20 µg of fibroblast extract. A one-site hyperbola line was generated for best-fit analysis using GraphPad.
Figure 2


(click to enlarge)

Figure 2. An example using MS131 to quantify Complex I levels using various concentration of human fibroblast extract.

Based on the above standard curve values, 13 µg of protein extract were loaded to each dipstick for the control sample (1) and the four unknown samples (2-5). Using GraphPad software, the signal intensity from the standard curve was interpolated and the quantity of Complex I in samples 1-5 was determined. Based on the above analysis, the unknown samples had 16% and 85% of Complex I levels compared to the control.


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Specifications
 
Data
 
Documentation
 
Citations

Downloadable Documents


   Technical Data Sheet

   Protocol

   Sample Preparation Guide


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Specifications
 
Data
 
Documentation
 
Citations

Published Studies Using This Product: Hong et al., 2010. Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts

Pennington et al., 2010. Differential effects of wild-type and A53T mutant isoform of alpha-synuclein on the mitochondrial proteome of differentiated SH-SY5Y cells.

Abstract #N1004, poster 667b, 13th Annual Conference on Retroviruses and Opportunistic Infections, 2006
"Mitochondrial Dysfunction in HAART Detected with Simple Quantitative Dipstick Assays for Mitochondrial Proteins."

Abstract and oral presentation at the United Mitochondrial Disease Foundation, June 14-17, 2006. Abstract in Mitochondrion, 2006, 6:282.
"Rapid lateral-flow "dipstick" immunoassays to detect and measure levels of assembled OXPHOS enzyme complexes in inherited and acquired mitochondrial disease."

Abstract #10C811. Presented in poster form at the 8th International Workshop on Adverse Drug Reactions and Lipodystrophy in HIV on the 24-26 September 2006. Abstract in Antiviral Therapy 2006; Supplement 3
"Mitochondrial dysfunction in HAART: Measurement of key OXPHOS enzymes with simple dipstick immunoassays."

Kashimshetty et al., 2009. Underlying mitochondrial dysfunction triggers flutamide-induced oxidative liver injury in a mouse model of idiosyncratic drug toxicity.

Nadanaciva et al., 2009. Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis.

Vercauteren et al., 2009. Short hairpin RNA-mediated silencing of PRC (PGC-1-related coactivator) results in a severe respiratory chain deficiency associated with the proliferation of aberrant mitochondria.

Shikuma et al., 2008. Mitochondrial oxidative phosphorylation protein levels in peripheral blood mononuclear cells correlate with levels in subcutaneous adipose tissue within samples differing by HIV and lipoatrophy status.


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Click here for a video demonstration of a dipstick assay.



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