Complex I Immunocapture monoclonal antibody

Catalog No. MS101c

$325.00 - 100 µg

Note: MS101c is a sample of pure immunocapture antibody, not immobilized to a solid support. For a ready-to-use immunocapture kit, containing the antibody crosslinked to agarose beads, please see MS101.


Product Specifications
Applications: Immunoprecipitation, Immunocytochemistry, In-Cell ELISA, Flow Cytometry
Species Reactivity: human, bovine, mouse, rat
Host Species: mouse
Isotype: IgG2b, κ
Clone ID: 18G12BC2
Concentration: 1 mg/mL in Hepes-Buffered Saline (HBS)-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 100 µg mAb can capture at least 25 µg complex I from 1 mg solubilized bovine heart mitochondria.
1 µg/mL for In-Cell ELISA (0.1 µg/well)
1 µg/mL for Flow Cytometry
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA


IP Images

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Figure 1. Complex I immunoprecipitation for antibody MS101c crosslinked to protein G-agarose beads as product MS101-250.
ICC Images

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Figure 2. Immunocytochemistry image of MS101c stained fibroblasts cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (18G12BC2, 1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 4884 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.
Flow Images

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Figure 3. HL-60 cells were stained with 1 µg/mL Complex I antibody (MS101c) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.



Downloadable Documents

   Technical Data Sheet

   Immunocapture Protocol

   Immunocytochemistry Protocol

   In-Cell ELISA Protocol

   Flow Cytometry Protocol

   MSDS Sodium Azide



Published Studies Using This Product: Willis et al., 2009. Isolated deficiencies of OXPHOS complexes I and IV are identified accurately and quickly by simple enzyme activity immunocapture assays.

Chinta et al., 2009. Reactive oxygen species regulation by AIF- and complex I-depleted brain mitochondria.

Wegrzyn et al., 2009. Function of mitochondrial Stat3 in cellular respiration.

Lund et al., 2008. Adenosine 3',5'-cyclic monophosphate (cAMP)-dependent phosphoregulation of mitochondrial complex I is inhibited by nucleoside reverse transcriptase inhibitors.

Doughan et al., 2007. Mitochondrial redox cycling of mitoquinone leads to superoxide production and cellular apoptosis.

Nadanaciva et al., 2007. Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays.

Qi et al., 2007. The mutant human ND4 subunit of complex I induces optic neuropathy in the mouse.

Keeney et al., 2006. Parkinson's disease brain mitochondrial complex I has oxidatively damaged subunits and is functionally impaired and misassembled.

Schilling et al., 2005. Rapid purification and mass spectrometric characterization of mitochondrial NADH dehydrogenase (Complex I) from rodent brain and a dopaminergic neuronal cell line.


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