Products for Complex I
(NADH ubiquinone oxidoreductase)
- Research Kits
- Dipstick Assay Kits
- Individual Antibodies
Overview
Complex I or NADH ubiquinone oxidoreductase is a large protein complex of 950,000 MW. made up by 45-46 different subunits. A total of 7 of the subunits of the complex are encoded on mtDNA, the remainder are nuclear encoded, made in the cytosol and translocated into the organelle for assembly at the inner membrane. The enzyme complex catalyses electron entry from NADH, as the name implies, via a flavin (FMN) and several non-heme iron centers. Complex I is sensitive to a wide range of inhibitors, many of which such as rotenone are pesticides or other common environmental toxins. Complex I dysfunction is a common cause of genetic OXPHOS defects. Altered functioning of this complex is also thought to contribute to several neurological disorders including Parkinsons disease and schizophrenia. Also there is evidence of complex I involvement in diabetes.
Research Kits
MS101 MitoProfile® Complex I Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~60 µg, ~120 µg or ~180 µg respectively of Complex I (NADH dehydrogenase) from heart mitochondria.
MS601 MitoProfile® Human Total OXPHOS Complexes Detection Kit
Contains a total of 360 µg or 720 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western blotting control.
MS602 MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
A cocktail of 220 µg antibodies comprised of six mAbs packaged individually. Each antibody is against a labile subunit of each of the 5 OXPHOS complexes and PDH. Each mAb is provided in a 50 µl aliquot at a 200x concentration, to allow reconstitution of each mAb in 10 ml working volume.
MS602a MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
Includes the same components as MS602 (above), but additionally contains 60 µg mAb against porin, and 120 µg against Complex V α subunit, and the required secondary antibodies for simultaneous 2 dye staining of samples.
MS604 MitoProfile® Rodent Total OXPHOS Complexes Detection Kit
Contains a total of 300 µg or 600 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from rodent sources. Mouse brain mitochondria are also included as a Western blotting control.
MSA31 MitoProfile® Dipstick Assay Kit for Apoptosis-Inducing Factor (AIF) Quantity
This kit contains 30 or 60 dipsticks and necessary components to quantify the amount of Apoptosis-Inducing Factor (AIF) from human sample material.
Dipstick Assay Kits
MS130 MitoProfile® Dipstick Assay Kit for Complex I Activity
Contains 30 or 60 dipsticks and necessary components for quantitative analysis of NADH ubiquinone oxidoreductase activity in human, bovine, rat, and mouse samples.
MS131 MitoProfile® Dipstick Assay Kit for Human Complex I Quantity
Contains 30 or 60 dipsticks and necessary components for quantitative analysis of NADH ubiquinone oxidoreductase in human and bovine samples.
MS133 MitoProfile® Dipstick Assay Kit for Rodent Complex I Quantity
Contains 30 or 60 dipsticks and necessary components for quantitative analysis of NADH ubiquinone oxidoreductase in mouse and rat samples.
Individual Antibodies
MS101c Complex I Immunocapture monoclonal antibody
100 µg monoclonal antibody which can immunocapture ~25 µg of Complex I (NADH dehydrogenase) from heart mitochondria.
MS103 Complex I subunit GRIM-19 monoclonal antibody
100 µg of monoclonal antibody reactive against the 19 kDa Complex I (NADH dehydrogenase) subunit GRIM-19. MS103 also labels GRIM-19 in immunocytochemical applications. Interestingly GRIM-19 is also present in the nucleus and cytoplasm. Its expression is necessary for interferonβ/retinoic acid induced tumor mortality.
MS104 Complex I subunit NDUFS4 monoclonal antibody
100 µg of monoclonal antibody reactive against the 18 kDa Complex I (NADH dehydrogenase) subunit NDUFS4.
MS105 Complex I subunit NDUFB8 monoclonal antibody
100 µg of monoclonal antibody reactive against the 20kDa Complex I (NADH dehydrogenase) subunit NDUFB8. Formerly listed as likely subunit ND6.
MS107 Complex I subunit NDUFB4 monoclonal antibody
100 µg of monoclonal antibody reactive against the 15 kDa Complex I (NADH dehydrogenase) subunit NDUFB4. MS107 also labels in immunocytochemical applications. Formerly listed as likely subunit NDUFA6.
MS108 Complex I subunit NDUFB6 monoclonal antibody
100 µg of monoclonal antibody reactive against the 17 kDa Complex I (NADH dehydrogenase) subunit NDUFB6.
MS109 Complex I subunit 8 kDa monoclonal antibody kDa
100 µg of monoclonal antibody reactive against an 8 kDa Complex I (NADH dehydrogenase) subunit.
MS110 Complex I subunit NDUFS3 monoclonal antibody
100 µg of monoclonal antibody reactive against the 30 kDa Complex I (NADH dehydrogenase) subunit NDUFS3. MS110 also labels NDUFS3 in immunocytochemical applications.
MS111 Complex I subunit NDUFA9 monoclonal antibody
100 µg of monoclonal antibody reactive against the 39 kDa Complex I (NADH dehydrogenase) subunit NDUFA9.
MS112 Complex I subunit NDUFS3 monoclonal antibody
100 µg of monoclonal antibody reactive against the 30 kDa Complex I (NADH dehydrogenase) subunit NDUFS3 in humans and C. elegans.
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MSA31 MitoProfile® Dipstick Assay Kit for AIF Quantity
The newest dipstick for rapidly and easily quantifying mitochondrial proteins. AIF is a multi-functional flavoprotein localized in the inter-membrane space that plays a major role in apoptosis, as well having an anti-oxidant role while also being an assembly factor for Complex I..
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