Products for Cancer Research
- Research Kits
- Individual Antibodies
Overview
Mitochondrial involvement in cancer is at several levels. Genetic alterations in OXPHOS genes can predispose to cancer, as in the case of succinate dehydrogenase subunit mutations, which cause paraganglioma syndromes.
Also, metabolic changes in energy metabolism are characteristic of cancer as tumors switch from an OXPHOS mode to glycolysis as source of ATP production. Further, the organelle is a key part of the signaling in apoptosis, a process that must be down regulated to allow cell proliferation in the disease.
Finally, recent work indicates that there is recruitment of mitochondrial proteins to the plasma membrane of cancer cells. This is shown in the figure above. The red spots distributed over the surface of the cell are ATP synthase molecules stained with mAb MS502 while the green is mitochondrial reticulum identified with matrix-located green fluorescent protein.
Research Kits
MS201 MitoProfile® Complex II Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of Complex II from heart mitochondria.
MS501 MitoProfile® ATP synthase (Complex V) Immunocapture Kit
250 µg, 500 µg or 750 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of ATP synthase (Complex V) from heart mitochondria.
MS601 MitoProfile® Human Total OXPHOS Complexes Detection Kit
Contains a total of 360 µg or 720 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western blotting control.
MS604 MitoProfile® Rodent Total OXPHOS Complexes Detection Kit
Contains a total of 300 µg or 600 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from mouse sources. Mouse brain mitochondria are also included as a Western blotting control.
MSA41 MitoProfile® Rapid Microplate Assay Kit for Cytochrome c Quantity
A 96-well activity microplate kit that is used to determine the amount of cytochrome c in a human, bovine, mouse, or rat samples. Includes detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Individual Antibodies
Monoclonal antibodies for staining ectopic (plasma membrane) ATP synthase:
MS502 ATP synthase (Complex V) subunit α monoclonal antibody
100 µg of monoclonal antibody reactive against the F1F0 ATP synthase (Complex V) α subunit. MS502 also labels α in immunocytochemical applications.
MS503 ATP synthase (Complex V) subunit β monoclonal antibody
100 µg of monoclonal antibody reactive against the F1F0 ATP synthase (Complex V) β subunit. MS503 also labels β in immunocytochemical applications.
MS504 ATP synthase (Complex V) subunit d monoclonal antibody
100 µg of monoclonal antibody reactive against the F1F0 ATP synthase (Complex V) d subunit. MS504 also labels the d subunit in immunocytochemical applications.
MS505 ATP synthase (Complex V) subunit OSCP monoclonal antibody
100 µg of monoclonal antibody reactive against the F1F0 ATP synthase (Complex V) OSCP subunit. MS505 also labels the OSCP subunit in immunocytochemical applications.
>MS506 ATP synthase (Complex V) subunit IF1 monoclonal antibody
100 µg of monoclonal antibody reactive against ATP synthase (Complex V) IF1 subunit. The antibody shows in Western blotting of whole mitochondria shows reactivity with two bands at 10 kDa and at 18 kDa, but in isolated F1F0 only the band at 10 kDa is observed (IF1). The 18 kDa band is the subunit IV of Complex IV.
MS508 ATP synthase (Complex V) subunit F6 monoclonal antibody
100 µg of monoclonal antibody reactive against the F1F0 ATP synthase (Complex V) F6 subunit. MS508 also labels the F6 subunit in immunocytochemical applications.
MS404 and MS407 reactive to the mitochondrially encoded subunit COX I and the nuclear encoded subunit COX IV respectively. These mAbs can be used in immunohistochemistry or in Western blots of excised tissue to identify tumor cells because the nuclear encoded COX IV is up regulated and the mitochondrially encoded COX I is downregulated in response to metabolic changes related to increased glycolytic potential of these cells. (ref. Hermann et al Proteomics 3. 1801-10 (2003)
MS404 Complex IV subunit I monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit I. MS404 also labels COX I in immunocytochemical applications.
MS407 Complex IV subunit IV monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit IV. MS408 also binds to COX IV.
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MS601 Human Total OXPHOS Complexes Detection Kit
This kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources.
The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used.
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