ATP synthase subunit OSCP monoclonal antibody
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Catalog No. MS505
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$325.00 - 100 µg
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UniProt Number:
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P48047
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Alternate Names:
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Complex V subunit OSCP, ATP synthase subunit O, Complex V subunit O, Oligomycin sensitivity conferral protein, ATP5O, ATPO
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Structure and Function:
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ATP synthase (complex V) is responsible for catalyzing the formation of ATP from ADP and Pi using energy from the proton concentration gradient created by preceding complexes of the respiratory chain. The two main components of this complex are F1 and F0, which are responsible for catalysis and transmembrane proton transport respectively.
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Product Specifications
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Applications:
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Western blotting, Immunocytochemistry (heat-induced antigen-retrieval improves signal), Immunohistochemistry, Flow Cytometry
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Species Reactivity:
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human, bovine
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Host Species:
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mouse
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Isotype:
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IgG1, κ
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Clone ID:
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4C11C10D12
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Concentration:
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1 mg/mL in Hepes-Buffered Saline (HBS)-Buffered Saline (HBS) with 0.02% azide as a preservative.
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Suggested Working Concentration:
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0.5 µg/mL for Western blotting
0.5-1 µg/mL for Immunocytochemistry
1 µg/mL for Flow Cytometry
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Storage Conditions:
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Store at 4°C. Do not freeze.
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Country of Origin:
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USA
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WB Images
(click to enlarge)
Figure 1. Isolated mitochondria from human heart (lane 1) and bovine heart (lane 2) detected with (MS505) anti-OSCP antibody.
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ICC Images
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Figure 2. Mitochondrial localization of ATP synthase visualized by immunocytochemistry using anti-ATP synthase subunit OSCP mAb 4C11C10D10 (MS505). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with MS505 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
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IHC Images
(click to enlarge)
Figure 3. Human colon tissue fixed with 4% PFA and antibody detection with MS505 using the ABC system. MS505 diluted 1:500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1:100 in 4% BSA in PBST) for 1 hour at room temp. Washed x3 in PBST and peroxidase activity was demonstrated using kit from Vector. Antigen retrieval was performed by 1 min pressure cooking 1mmol EDTA pH8.
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Flow Images
(click to enlarge)
Figure 4. HL-60 cells were stained with 1 µg/mL ATP synthase subunit OSCP antibody (MS505) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Published Studies Using This Product:
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Cameron et al., 2010. Complex V TMEM70 deficiency results in mitochondrial nucleoid disorganization.
Mayr et al., 2010. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 {varepsilon} subunit.
Giorgio et al., 2009. Cyclophilin D modulates mitochondrial F0F1-ATP synthase by interacting with the lateral stalk of the complex.
Sansanwal et al., 2009. Caspase-4 may play a role in loss of proximal tubules and renal injury in nephropathic cystinosis.
Aubert et al., 2008. The antiapoptotic herpes simplex virus glycoprotein J localizes to multiple cellular organelles and induces reactive oxygen species formation.
Stiburek et al., 2007. Knockdown of human Oxa1l impairs the biogenesis of F1Fo-ATP synthase and NADH:ubiquinone oxidoreductase.
Margineantu et al., 2007. Hsp90 inhibition decreases mitochondrial protein turnover.
Contessi et al., 2007. IF(1) distribution in HepG2 cells in relation to ecto-F(0)F (1)ATPsynthase and calmodulin.
Cortés-Hernández et al., 2007. ATP6 homoplasmic mutations inhibit and destabilize the human F1F0-ATP synthase without preventing enzyme assembly and oligomerization.
Shin et al., 2005. Down-regulation of mitochondrial F1F0-ATP synthase in human colon cancer cells with induced 5-fluorouracil resistance.
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