250 µg, 500 µg or 750 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 µg, ~250 µg or ~500 µg respectively of ATP synthase (Complex V) from heart mitochondria.

The ATP synthase Immunocapture Kit allows isolation of the ATP synthase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for the ATP synthase (Complex V) immunocapture kit include but are not limited to examining alterations of Complex V subunits in inherited mitochondrial diseases, Alzheimer's disease, angiogenesis, hypertension and aging.

Note: The immunocapture protocol for this kit requires MitoSciences detergent (lauryl maltoside, MS910).

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