ATP synthase Immunocapture monoclonal antibody

Catalog No. MS501c

$325.00 - 100 µg

Note: MS501c is a sample of pure immunocapture antibody, not immobilized to a solid support. For a ready-to-use immunocapture kit, containing the antibody crosslinked to agarose beads, please see MS501.



 
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Product Specifications
 
Applications: Immunoprecipitation
Species Reactivity: human, bovine, mouse
Host Species: mouse
Isotype: IgG2b, κ
Clone ID: 12F4AD8AF8
Concentration: 1 mg/mL in Hepes-Buffered Saline (HBS) with 0.02% azide as a preservative.
Suggested Working Concentration: 100 µg mAb can capture at least 50 µg Complex V (determined with 1 mg solubilized bovine heart mitochondria)
Storage Conditions: Store at 4°C. Do not freeze.
Country of Origin: USA


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Figure 1




Figure 1. Using the immunoprecipitation protocol provided, ATP synthase was isolated from various samples by antibody MS501c crosslinked to protein G-agarose beads (MS501-250). Protein band identities were confirmed by mass spectrometry.


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Downloadable Documents


   Technical Data Sheet

   Immunocapture Protocol


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Published Studies Using This Product: Loeb et al., 2010. The Transgenic Overexpression of α-Synuclein and Not Its Related Pathology Associates with Complex I Inhibition.

Chen et al., 2009. Oxygen consumption can regulate the growth of tumors, a new perspective on the Warburg effect.

Will et al., 2008. Effect of the multitargeted tyrosine kinase inhibitors imatinib, dasatinib, sunitinib, and sorafenib on mitochondrial function in isolated rat heart mitochondria and H9c2 cells.

Dykens et al., 2008. In vitro assessment of mitochondrial dysfunction and cytotoxicity of nefazodone, trazodone, and buspirone.

Nadanaciva et al., 2007. Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assays.


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