Products for Alzheimer's Research
- Microplate Assay Kits
- Dipstick Assay Kits
- Research Kits
- Individual Antibodies
Overview
The involvement of mitochondria in Alzheimer's Disease has been known for many years with reduced cytochrome c oxidase assembly and activity considered as an early event in the disease, and reduced cytochrome c oxidase activity has been reported in platelets from patients with Alzheimer's Disease.
Recent studies begin to explain mitochondrial involvement. The Alzheimer's amyloid precursor protein has now been localized to mitochondria as has the toxic amyloid beta peptide. The binding site for amyloid beta has been identified as alcohol dehydrogenase in the matrix space of the organelle. This enzyme can metabolize aldehydes and if the function is impaired could be involved in generation of oxidative radicals and a consequent cell death. Another mitochondrial protein implicated in Alzheimer's Disease is pyruvate dehydrogenase.
MitoSciences has research kits and mAbs specific to several of the proteins above.
Microplate Assay Kits
MS441 MitoProfile® Rapid Microplate Assay Kit for Human Complex IV Activity
A rapid activity microplate kit that is used to determine the activity of cytochrome c oxidase in a human sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS442 MitoProfile® Rapid Microplate Assay Kit for Human Complex IV Quantity
A rapid activity microplate kit that is used to determine the amount of cytochrome c oxidase in a human sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS443 MitoProfile® Rapid Microplate Assay Kit for Human Complex IV Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both the activity and quantity of cytochrome c oxidase in a human sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS444 MitoProfile® Rapid Microplate Assay Kit for Mouse Complex IV Activity
A 96-well activity microplate kit that is used to determine the activity of cytochrome c oxidase in a mouse sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS445 MitoProfile® Rapid Microplate Assay Kit for Mouse Complex IV Quantity
A 96-well activity microplate kit that is used to determine the amount of cytochrome c oxidase in a mouse sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS446 MitoProfile® Rapid Microplate Assay Kit for Mouse Complex IV Activity and Quantity (Multiplexing)
A 96-well multiplexing microplate kit that is used to determine both the activity and quantity of cytochrome c oxidase in a mouse sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
MS447 MitoProfile® Rapid Microplate Assay Kit for Rat Complex IV Activity
A 96-well activity microplate kit that is used to determine the activity of cytochrome c oxidase in a rat sample with greater speed and simplicity. Includes buffers, detergent, substrates, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Dipstick Assay Kits
MS430 MitoProfile® Dipstick Assay Kit for Human Complex IV Activity
Contains 30 or 60 dipsticks and necessary components for quantitative analysis of cytochrome c oxidase activity in human and bovine samples.
MS431 MitoProfile® Dipstick Assay Kit for Human Complex IV Quantity
Contains 30 or 60 dipsticks and necessary components for quantitative analysis of cytochrome c oxidase in human and bovine samples.
MS432 MitoProfile® Dipstick Assay Kit for Rodent Complex IV Activity
Contains 30 or 60 dipsticks and necessary components for quantitative analysis of cytochrome c oxidase activity in mouse and rat samples.
Research Kits
MS401 MitoProfile® Complex IV Immunocapture Kit
250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex IV from heart mitochondria.
MS601 MitoProfile® Human Total OXPHOS Complexes Detection Kit
Contains a total of 360 µg or 720 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria are also included as a Western blotting control.
MS602 MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
A cocktail of 220 µg antibodies comprised of six mAbs packaged individually. Each antibody is against a labile subunit of each of the 5 OXPHOS complexes and PDH. Each mAb is provided in a 50 µl aliquot at a 200x concentration, to allow reconstitution of each mAb in 10 ml working volume.
MS602a MitoProfile® OXPHOS/PDH Immunocytochemistry Kit
Includes the same components as MS602 (above), but additionally contains 60 µg mAb to porin, and 120 µg to Complex V α subunit, and the required secondary antibodies for simultaneous 2 dye staining of samples.
MS604 MitoProfile® Rodent Total OXPHOS Complexes Detection Kit
Contains a total of 300 µg or 600 µg of antibodies and is a premixed solution of 5 monoclonal antibodies against a subunit of each of the 5 OXPHOS complexes in mitochondrial preparations from rodent sources. Mouse brain mitochondria are also included as a Western blotting control.
MSP01 MitoProfile® PDH Immunocapture Kit
250 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg of PDH complex from heart mitochondria.
Individual Antibodies
MS404 Complex IV subunit I monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit I. MS404 also labels COX I in immunocytochemical applications.
MS405 Complex IV subunit II monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit II. MS405 also labels COX II in immunocytochemical applications.
MS406 Complex IV subunit III monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (complex IV) subunit III.
MS407 Complex IV subunit IV monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit IV. Reacts with human, bovine, mouse and rat. MS408 also binds COX IV.
MS408 (alternate to 407) Complex IV subunit IV monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit IV. MS408 also labels COX IV in immunocytochemical applications. MS407 also binds COX IV. Reacts only with human and bovine, but better than MS407 for immunocytochemistry in these species.
MS409 Complex IV subunit Va monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit Va. MS409 also labels COX Va in immunocytochemical applications.
MS410 Complex IV subunit Vb monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (complex IV) subunit Vb.
MS414 Complex IV subunit VIc monoclonal antibody
100 µg of monoclonal antibody reactive against the COX (Complex IV) subunit VIc.
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Complex IV Rapid Microplate Assay Kit
This kit is used to determine both the activity and quantity of Cytochrome c oxidase in a human sample. The ratio of the two is a measure of specific activity.
The COX enzyme is immunocaptured within the wells of the microplate and the activity is determined by following the oxidation of reduced Cytochrome c colorimetrically by the absorbance change at 550 nm.
The amount can then be determined in these same wells by adding a COX specific detector antibody and the appropriate alkaline phophatase conjugated antibody.
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